Isolation and characterisation of the potent broad spectrum β-lactamase AME 1 from Streptomyces antibioticus ssp. Antibioticus
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منابع مشابه
Multiple Cellulase System from Streptomyces Antibioticus.
Enger, M. D. (North Dakota State University, Fargo), and B. P. Sleeper. Multiple cellulase system from Streptomyces antibioticus. J. Bacteriol. 89:23-27. 1965.-Starch-block zone electrophoresis was used to isolate five electrophoretically distinct, active cellulolytic components (I to V) from the crude extracellular cellulase system of Streptomyces antibioticus (strain C2A). Agar diffusion prec...
متن کاملIsolation of flavohemoglobin from the actinomycete Streptomyces antibioticus grown without external nitric oxide stress.
A flavocytochrome protein was isolated from the actinomycete Streptomyces antibioticus. The purified protein contained protoheme and FAD, and its M(r) was estimated to be 52000. The absorption spectra in its resting oxidized, dithionite-reduced, carbon monoxide-bound, and oxygenated (O(2)-bound) forms were characteristic of those of flavohemoglobin (Fhb). The N-terminal amino acid sequence show...
متن کاملIncorporation of sarcosine into the actinomycins synthesized by Streptomyces antibioticus.
Sarcosine is an amino acid apparently not widely distributed in Nature. As a free amino acid, sarcosine has been demonstrated in the radial caeca of the starfish Astropecten aurantiacus (Kossel & Edlbacher, 1915), in the muscles of Elasmobranchii (Tarr, 1958) and in the reindeer moss, Cladonia itlvatica (Linko, Alfthan, Miettinen & Virtanen, 1953). Sarcosine has been found bound in peptides in ...
متن کاملMorphological changes accompanying actinomycin production in Streptomyces antibioticus.
The fine structure of the actinomycin producing bacterium Streptomyces antibioticus has been studied. The typical appearance of freshly ineculated cells is one of a generally electron dense cytoplasm. As cellular protein synthesis ceases and secondary metabolism (actinomycin biosynthesis) is initiated, a gradual loss of cytoplasmic density occurs, revealing in many cells membrane enclosed vesic...
متن کاملCloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.
In two separate studies a BclI-generated DNA fragment coding for the enzyme tyrosinase, responsible for melanin synthesis, was cloned from Streptomyces antibioticus DNA into two SLP1.2-based plasmid vectors (pIJ37 and pIJ41) to generate the hybrid plasmids, designated pIJ700 and pIJ701, using S. lividans 66 as the host. The fragment (1.55 kb) was subcloned into the multicopy plasmid pIJ350 (whi...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1983
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1983.tb00270.x